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HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

FeFAB cells (a kind gift from Dr Martin Löfefabchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。

The molecular clone of feline foamy virus (pCF-7) and FeFAB cell for FeFAB assay were kind gifts from Dr. Martin Lochelt, German Cancer Research Center, Germany.

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